Muhammad Asad Uz Zaman has completed his B.Pharm. and M.Pharm. from Department of Pharmaceutical Chemistry, University of Dhaka. Abstract of his thesis was orally presented in 39th Annual meeting of Environmental mutagen society of India (EMSI) on “Biotechnological Advances in Environmental Health and Biodiversity Conservation (EHBC)” at Manipur University on May, 2015. He was awarded National Science and Technology Fellowship (2014-2015) provided by Ministry of Science & Technology, Government of Bangladesh. He is a Registered Pharmacist (Grade – A) under the Pharmacy Council of Bangladesh. He is currently working as a product development officer in ACI (Advanced Chemical Industries) Pharmaceuticals Ltd.
Thrombosis leads to myocardial infarction, stroke and other cardiovascular complications. Thrombolytic agents such as t-PA, u-PA, streptokinase etc are used to treat complications related to thrombosis. However, investigations are being pursued to find out new microbial enzymes as thrombolytics having better efficacy and specificity with less side effects, availability and affordability. To search for new thrombolytic proteases from microbial sources, two mutant strains of Bacillus licheniformis MZK05M9 and Bacillus licheniformis EMS250-O-1 were cultured in modified Urea-glucose and Urea-molasses medium at 370C under shake culture conditions yielding 840.112 units/mg and 1128.992 units/mg respectively. The enzymes were partially purified using ammonium sulfate precipitation and ultrafiltration yielding 37713.922 units/mg from MZK05M9 and 40129.916 units/mg from EMS250-O-1. The molecular weight of the partially purified enzymes from the strain MZK05M9 was approximately 27.2 kDa and purification increased its specific activity to 16.49 fold with a recovery of 10%, whereas the same from the strain EMS250-O-1 was approximately 25.5 kDa with an increase in 12.28 fold having a recovery of 17.8%. The partially purified protease enzymes exhibited 32.84% and 38.01% thrombolytic activity for strain MZK05M9 and EMS250-O-1 respectively, by in-vitro clot lysis assay. The present results will be an useful basis for development of viable thrombolytic drugs to prevent or cure thrombosis and related disorders.
Samer M. Al-Hulu, Assistant Professor of Microbiology, has completed his PhD at the age of 29 years from Babylon University/College of Science. He has published more than 14 papers in microbiology field. Al-Hulu, has training at Ministry of Health at Laboratory of Babylon Maternity and Children Hospital. Now working at Al-Qasim Green University/College of Food Science.
Quorum sensing (QS) is a process which included communication between bacterial cells, also involved production, deletion and response for extracellular signaling molecules which called autoinducer (AI). AI due to accumulation of bacterial and the density increased and changing in their cell number and altering in gene expression. Quorum sensing control genes having more benefit when groups of bacteria acting in synchrony. There are three types of quorum sensing system which includes LuxI/LuxR –type which found in gram negative bacteria which using acyl-homoserine lactones (AHL) as a signal molecules, oligopeptide –two component-type which found in gram positive bacteria which using small peptide as signal molecules, and LuxS encoded autoinducer 2 (AI-2) quorum sensing which found in both gram positive and gram negative bacteria. There are many examples about biofilm formation by Qs system such as Lux-dependent system which mediate biofilm formation in S.pneumonia, Com CDE system which responsible for biofilm formation in Streptococcus mutans, AI play an important role in oral cavity biofilm for Streptococcus gordonii as well as the etiology of dental caries Streptococcus mutans. AI stimulate biofilm formation and changes its architecture by stimulating flagellar motility via quorum sensing regulator MqsR which act through the two component motility regulatory system QseBC.The recent finding showed that AI-2 regulate biofilm formation in Actinomycetemcomitans, though QseB system. The formation of biofilm in P.aeruginosa is coordinated by QS pathway which involves the transcriptional regulator LasR and RhIR system and the signal QS molecules:C12HSL(N-C3-oxododecanoyl-HSLlactone)=OdDHL) andC4-HSL [ N-butanoyl-L-HSL].