Gun-Woo Oh
Pukyong National University, Republic of Korea
Title: Fabrication, characterization and determination of biological activities of poly (ε-Caprolactone)/chitosan-caffeic acid composite nano/microfiber mat for wound dressing application
Biography
Biography: Gun-Woo Oh
Abstract
Electrospinning In the present study, we designed composite nano/microfiber mats consisting of poly (ε-caprolactone) (PCL), chitosan (CH), or chitosan-caffeic acid conjugate (CCA) fabricated by an electrospinning technique for wound dressing application. The average diameters of PCL, PCL/CH, and PCL/CCA composite nano/microfiber mats are 1.30±1.07, 1.20±1.22, and 0.94±0.68 μm, respectively. Based on UTM analysis, the PCL/CCA composite significantly increases tensile properties compared with the PCL and PCL/CH composites. Additionally, initial cell attachment and cell proliferation of the composites using neonatal human dermal fibroblast (NHDF-neo cells), as well as the anti-microbial effect against Staphylococcus aureus, was investigated. The PCL/CCA composite shows significantly higher initial cell attachment and cell proliferation than the PCL and PCL/CH composites, and a high anti-microbial effect was observed compared to the PCL and PCL/CH composites. Based on these results, the CCA is demonstrated to be good supplemental bioactive agent for wound dressing applications and skin tissue engineering.