Seong-Yeong Heo
Pukyong National University, Republic of Korea
Title: Fabrication and characterization for promoting osteoblast differentiation using bioactive substance from fish frame
Biography
Biography: Seong-Yeong Heo
Abstract
In order to utilize fish byproducts in a calcium and phosphate supplement, Johnius belengerii frame composed of flesh and skeleton discarded from industrial processing were degraded by pepsin in acetic acid solution. In current study, we report the osteogenic effect of fish frame extracts (FBE) from Johnius belengerii in MC3T3-E1 pre-osteoblast. We designed composite scaffolds consisting of poly-caprolactone (PCL) and FBE fabricated by three axis plotting system for bone regeneration, The effect of FBE coated scaffolds (1 and 3%) on various mechanical properties and charactertistics including the morphology image, FT-IR analysis, tensile properties and drug releases were investigated. Moreover, the in vitro biocompatibilities of the FBE coated scaffolds were examined using MC3T3-E1 pre-osteoblast. On the scaffold surface, cell attachment, proliferation, mineralization and osteogenic factors were determined. At the results, the PCL/FBE combined scaffolds showed cell attachment, proliferation, mineralization and osteogenic factors than the PCL scaffold. Consequently, the FBE combined scaffold suggents further investigation a potential biomedical engineering field due to enhancement of osteogenesis.