5^th Global Pharmacists Annual Meeting July 14-15, 2016 Brisbane, Australia

Seok-Chun Ko has completed his PhD from Jeju National University and Post-doctoral studies from Pukyong National University School of Biomedical Enginnering. He is the Research Professor of marine-integrated bionics Center. He has published more than 35 papers in SCI journals and has been serving as an Editorial Board Member of repute.

To prepare antioxidative peptide from Styela plicata, nine proteases were employed for enzymatic hydrolysis, and the antioxidative activities of the hydrolysates were investigated using electron spin resonance (ESR) spectrometer. Among the hydrolysates, tryptic hydrolysate exhibited the highest antioxidative activities than those of other enzymatic hydrolysates. In order to purity a peptide having potent antioxidative properties, tryptic hydrolysate was separated using consecutive chromatographic methods, and andtioxidative peptide was identifi ed to be Leu-Pro-His-Pro-Ser-Phe (696.3 Da) by Q-TOF ESI mass spectroscopy. It scavenged peroxyl, hydrolxyl and DPPH radicals at the IC50 values of 0.05, 1.98 and 0.17 mM, respectively. Pretreatment with the purifi ed peptide decreased the death of AAPH-treated cells, and reduced the generation of intracellular reactive oxygen species (ROS) in a dose-dependent manner in AAPH-treated cells. Furthermore, the purifi ed peptide signifi cantly reduced ROS generation and cell death in zebrafi sh model. Th ese results indicate that enzymatic hydrolysates of S. plicata protein possess potent antioxidative activity.

Van-Tinh Nguyen received his PhD from the Department of Biomedical Engineering, Pukyong National University, Korea. He graduated in 2012 from the University of Chosun and his current research interests include the isolation, safety and bioavailability of bioactive materials; development of marine-integrated cells and tissue regenerative biomedical substances.

This study examined the eff ects of Ciona intestinalis calcitonin-like peptide (CCLP) on osteoblast diff erentiation and mineralization in the culture system of MC3T3-E1 cells. Th e primary structures of the CCLP containing Cys-Asp-Gly-Val- Ser-Th r-Cys-Trp-Leu-His-Glu-Leu-Gly-Asn-Ser-Val-His-Ala-Th r-Ala-Gly-Gly-Lys-Gln-Asn-Val-Gly-Phe-Gly-Pro-NH2 was synthesized automatically using the solid phase method with fl uorenylmethoxycarbonyl (Fmoc) resin. Pre-osteoblast MC3T3-E1 cells were cultured with various concentrations of CCLP (7.5, 15, and 30 μM) during the osteoblast diff erentiation period. To examine osteoblast diff erentiation, alkaline phosphatase (ALP) activity was determined by reading the absorbance at 405 nm using a spectrophotometer, and mineralization was evaluated by staining with Alizalin red S. Moreover, the expression of diff erentiation markers such as ALP, osteocalcin (OSC), and osteopontin (OPN) were measured using RT-PCR and Western blot analysis. Th e results showed that CCLP did not exhibit any cytotoxic eff ect on MC3T3-E1 cells even at the highest concentration (30 μM) at 2 and 5 days. CCLP also enhanced MC3T3-E1 cells proliferation, diff erentiation, and mineralization demonstrated by the increased expression of several osteoblast phenotype markers such as ALP, and Alizarin red S staining. In addition, the CCLP induced mitogenactivated protein kinase (MAPK) pathway in MC3T3-E1 cells. Th ese results suggest that CCLP exerts positive eff ects on osteoblast diff erentiation and may represent a potential target for pharmaceutical development.

Seong-Yeong Heo has completed his MSc at the 2016 from Pukyong National University in Korea. He has studied in marine life science and tissue engineering. Now, he is performing that the tissue regenerative scaffold fabricated by three axis plotting system and cell signaling investigated by western blot and RT-PCR analysis. Additionally, he has published 3 papers in reputed journals.

Annually, large amounts of fi shery by-products such as bone, skin, fi ns, internal organs and head from seafood inductrial processing are discarded or manufactured into fi sh feed. So, seafood by-products are needed to convert profi table and marketable products. However, studies on the utilization of organic components or minerals in the fi sh organs are scarce. Th erefore, many researchers have been performed to utilize the abundance of protein, carbohydrate and mineral from fi sh organs. Th e purpose of this study was to evaluate the potential therapeutic properties of the pepsin extract of the Johnius belengerii frame against lipopolysaccharide (LPS) stimulated macrophage. Th e fi sh bone extracts (FBE) signifi cantly inhibited the nitric oxide (NO) production and the induced dose dependent reduction of the protein and mRNA elvels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2). Additionally, the FBE reduced the mRNA levels of infl ammatory cytokines, including tumor necrosis factor-α, interleukin (IL) -1β, IL-6. Based on the results, the FBE inhibits LPS-induced infl ammation via blocking the iNOS, COX-2, and infl ammatory cytokines in macrophages.

Gun-Woo Oh has studied in Interdisciplinary Program of Biomedical, Mechanical & Electrical Engineering from Pukyoung National University in Busan, Korea. He also has worked in marine biomedical science lab where he is able to experiment constantly on cells, synthesis, extraction and others, and the adviser is Won-Kyo Jung and then he applied doctoral course in same University and lab.

In various biomedical application including tissue engineering and drug delivery system (DDS), poly (ε-caprolactone) (PCL) has been commonly used as an excellent bioresorbable and biocompatible polymer. Phlorotannins, which a subgroup of tannins, are known to have various biological activities such as anti-infl ammation, anti-oxidant and anti-cancer. Th e purpose of the present study was to fabricate phlorotannin-coated PCL fi lm for using biomedical engineering. Fabrication of fi lms were used by solvent casting technique and coating method. Briefl y, PCL fi lm was coated by phlorotannin solution (1, 3 and 5 wt%) and then mechanical characterization of fabricated fi lm was investigated through Fourier Transform Infrared Spectroscope (FT-IR), universal testing machine (UTM) and water-contact angle. Furthermore, cytotoxicity and anti-infl ammation of fi lm were investigated by using the MTT assay and nitric oxide production assay on LPS-stimulated macrophage RAW264.7 cells, respectively. Base on water-contact angle, phlorotannincoated PCL fi lm was more highly hydrophilic surface compared with non-coated PCL fi lm. In addition, phlorotannin-coated PCL fi lm did not cytotoxicity and signifi cantly inhibited the nitric oxide production. Th e results of this study suggested that phlorotannincoated PCL fi lm is potentially useful as a bioactive ingredient in tissue engineering and DDS fi elds.