Khaled Magdy Sadek
Mansoura University, Egypt
Title: Role of Oxidative Stress, Neuroprotective Heat Shock Protein 70 and Connexin 43 in Pentylenetetrazole-induced Seizures in Rats: Possible Protective Effect of Ferulic Acid
Biography
Biography: Khaled Magdy Sadek
Abstract
Objective: the present study aimed to investigate the role of Cx43 and Hsp70 in PTZ induced seizures and the effects of ferulic acid on in rat model of PTZ-induced epilepsy. Methods: twenty four Sprague dawely rats were subdivided into 4 equal groups, a) normal group (normal rats), b) PTZ group: rats received PTZ at a dose of 50 mg/kg i.p. on alternate day till the animal became fully kindled, c) FA before group, rats received FA at a dose of 40 mg/kg daily via gastric gavage with PTZ on alternate day d)FA after group, rats FA at a dose of 40 mg/kg daily via gastric gavage from 6th dose of PTZ. The parameters measured were the Racine score, seizure duration and latency to develop kindling, immunohistopathological study for Cx43 and Hsp70 expression in hippocampus and hippocampal lipid peroxidation studies. Results: In this study, PTZ caused significant increase in oxidative stress marker (MDA), Cx43 expression and Hsp70 expression in hippocamal region with significant reduction in antioxidants GSH and CAT in hippocampal region. FA pretreatment significantly reduced the seizure severity and duration kindling in PTZ treated rats and exhibited a marked prolongation in the latencies to seizures. Also, FA pretreatment caused significant improvement in markers of oxidative stress, Cx43 expression and Hsp70 expression in hippocampal region. Although FA post-treatment caused significant improvement in seizure behavior and oxidative stress and HSP70, it did not cause any significant change in Cx43 compared to PTZ group. Conclusions: These findings may provide insights into the understanding of the mechanism of FA as an anti-kindling agent and could offer a useful support to the basic antiepileptic therapy in preventing the development of PTZ induced seizures, suggesting its potential for therapeutic applications in temporal lobe epilepsy.