Afaf I. Shehata
King Saud University, Saudi Arabia
Title: Molecular detection and characterization of Fusarium sporotrichioides based on ITS2 rDNA polymorphism
Biography
Biography: Afaf I. Shehata
Abstract
The genus Fusarium contains a number of soil borne species with worldwide distribution The presented PCR assays are highly selective and sensitive in detecting the Fusarium genus. In order to identify the eighteen Fusarium isolates obtained at the molecular level, PCR analysis using primer specific for the conserved ITS DNA region of Fusarium genus was conducted. The data indicated that, all of the eighteen isolates showed a clear band corresponding to the expected molecular size of the ITS region (431bp). These results confirmed that all the tested samples belong to the genus Fusarium. Also, when all eighteen isolates of Fusarium species were analyzed by PCR for fumonisin producing ability using FUM1 gene based primers, the expected DNA fragment of 183 bp was amplified only in Fusarium verticillioides ( 3 isolates), Fusarium avenaceum ( 3 isolates) , Fusarium semitectum ( 1 isolate) and Fusarium culmorum ( 2 isolates) showed a positive result with FUM1 gene set of primers. No bands were seen in other isolates of Fusarium spp. and the standard ( Fusarium graminearum ). The genus Fusarium contains a number of soil borne species with worldwide distribution The presented PCR assays are highly selective and sensitive in detecting the Fusarium genus. In order to identify the eighteen Fusarium isolates obtained at the molecular level, PCR analysis using primer specific for the conserved ITS DNA region of Fusarium genus was conducted. The data indicated that, all of the eighteen isolates showed a clear band corresponding to the expected molecular size of the ITS region (431bp). These results confirmed that all the tested samples belong to the genus Fusarium. Also, when all eighteen isolates of Fusarium species were analyzed by PCR for fumonisin producing ability using FUM1 gene based primers, the expected DNA fragment of 183 bp was amplified only in Fusarium verticillioides ( 3 isolates), Fusarium avenaceum ( 3 isolates) , Fusarium semitectum ( 1 isolate) and Fusarium culmorum ( 2 isolates) showed a positive result with FUM1 gene set of primers. No bands were seen in other isolates of Fusarium spp. and the standard ( Fusarium graminearum )