Gazi Md Mustakim
North South University, Bangladesh
Title: Anti-hyperglycemic activity of Aegle marmelos is partly mediated by alpha amylase inhibition and retardation of glucose absorption
Biography
Biography: Gazi Md Mustakim
Abstract
The study was designed to observe the anti-hyperglycemic effect of Agele marmelos as reported earlier in diabetic model rats. This study was carried out to explore the possible effects of A. marmelos extract on carbohydrate absorption and glucose utilization. We measured fasting blood glucose and performed glucose tolerance test to evaluate the primary anti-hyperglycemic effect, in type 2 diabetic rats. Further, we studied the plasma insulin concentration and serum glucose level. Effect of extracts on carbohydrate break down, sucrose malabsorption and gut perfusion study of the GI tract and α-amylase inhibition were assessed. Gastrointestinal motility was seen by BaSO4 milk traverse test. Treatment of extracts suppressed blood glucose elevation after oral sucrose (2.5 g/kg) administration and (1.25 g/kg) significantly improved oral glucose tolerance in type 2 diabetic rats. A. marmelos extracts showed significant changes in plasma insulin secretion. The extracts significantly reduced glucose absorption in the in situ perfused rat intestinal model at two different doses. The extract inhibited the action of α-amylase, and this study was confirmed again by the sucrose malabsorption test, where sucrose digestion was inhibited throughout the length of the GI Tract. During the chronic study, body mass of rats returned to normal and their polydipsic and polyphagic conditions were improved too. This combination of in vitro, in vivo and in situ intestinal perfusion technique confirmed the anti-hyperglycemic activity of A. marmelos and its tissue level mechanism. Additional study is required to fully demonstrate the effects of the active compounds to the precise mechanism of glucose-fiber binding capacity and glucose transporters.