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The Beautiful Skin Institute PLLC, USA
Dr. Esche is a board-certified dermatologist and founder of The Beautiful Skin Institute in Northern Virginia. He has received more than 10 academic awards for his research, and has authored more than 70 scientific publications. Dr. Esche became the National Winner of the Doctor’s Choice Award in Dermatology in 2014 and again in 2015
The increasing demand for cosmetic facial rejuvenation with minimal risk and no downtime has led a surge in minimally invasive procedures. However, injection of both neuromodulators and fillers may result in bruising even in experienced injectors.
The AccuVein bruise prevention device (AccuVein Inc., Huntington, NY) helps minimize bruising following injectable treatments by visualizing veins that otherwise cannot be seen by the naked eye. The hand-held 9.7 ounce vein illuminator projects a steady and safe infrared beam onto the skin. The light is absorbed by hemoglobin and reflected by surrounding tissue.
The competing VeinViewer (Christie Medical Holdings, Inc., Memphis, TN) appears to represent a feasible alternative for minimally invasive facial rejuvenation, while the Veinlite vein finder (Translite LLC, Sugarland, TX) is more suitable for venipuncture mostly due to its smaller area of illumination. The Site-Rite Prevue Ultrasound System (C. R. Bard Inc., Murray Hill, NJ) is also more suitable for peripheral venous access.
We use the AccuVein device mostly for neuromodulator treatment in the lateral canthal region and for filler injection in the tear trough, nasolabial folds and lips.
In summary, both Accuvein and VeinViewer appear to be useful accessories for cosmetic facial injections.
In conclusion, vein illumination will likely become a new standard of care for minimally invasive rejuvenation
SV Clinic And Laser, Cambodia
Kea Hangsovann is an owner of SV Clinic and Laser, Cambodia. He is a member of International Peeling Society, Swiss Academy of Cosmetic Dermatology and Aesthetic Medicine (SACDAM) and Endopeel user. He is an Aesthetic Medical Doctor, specializes in “Acne treatment, chemical peeling, scar treatment, filler, endopeel, skin disease, mesotherapy, allergy dermatitis, anti-aging, and laser”
Chemical peeling, also termed chemexfoliation, represents accelerated exfoliation or skin damage induced by caustic agents that cause controlled damage, followed by the release of cytokines and inflammatory mediators, resulting in thickening of the epidermis, deposition of collagen, reorganization of structural elements, and increases in dermal volume. This process decreases solar elastosis and replaces and reorients the new dermal connective tissue. The result is an improved clinical appearance of the skin, with fewer rhytides and decreased pigmentary dyschromia, and a more youthful appearance
- Dermatological Diseases | Aesthetic and Cosmetic Dermatology | Dermatology: Therapies and Advances | Clinical and Medical Dermatology | Hair and Nails | Melanoma
Location: Bleriot 2
The Beautiful Skin Institute PLLC, USA
Meguro Chen Dermatology Clinic, Japan
Hui-Min David Wang, a Full Professor at Graduate Institute of Biomedical Engineering (National Chung Hsing University), graduated from the Department of Chemical Engineering, National Cheng Kung University, Tainan, Taiwan. In 2014, he got Ta-You Wu Memorial Award which is the highest price to young scientist of Ministry of Science and Technology (MOST) in TW. In 2015, he got Young Scholars Biotechnology Invention Award which is the highest price to young scientist of Taiwan Society of Biochemistry and Molecular Biology (TSBMB) in TW. In 2016, he got the Precious Stone Award in TW
This study assessed the use of astaxanthin as an anticancer agent for increasing inhibition to melanomacells (A375 and A2058). Wound healing and invasion assays presented that astaxanthin treatmentreduced melanoma cell migration in a dose-dependent manner. The effects on melanoma cell migrationwere conferred via suppressed expressionsof matrix metalloproteinases 1, 2 and 9. Dichlorofluoresceindiacetate assay further showed that astaxanthin treatment reduced production of cellular reactiveoxygen species. Cellular proliferation assay revealed potent dose-dependent inhibiting effects onmelanoma cells. One-dimensional flow cytometric analysis demonstrated that astaxanthin induced cellcycle arrest in G1 phase. Mechanisms of apoptosis were verified by double fluorescence staining withannexin V-fluorescein isothiocyanate and propidium iodide. The antitumor effects of astaxanthin significantlydecreased tumor size in a xenograft model. In summary, the experimental results showed thatastaxanthin has potent in vivo and in vitro inhibiting effects on melanoma tumor growth for developingas chemotherapeutic agents.
Equisetum ramosissimum, a genus of Equisetaceae, is a medicinal plant that can be separated into ethyl acetate (EA), dichloromethane (DM), n-hexane (Hex),methanol (MeOH), andwater extracts. EAextract was known to have potent antioxidativeproperties, reducing power, DPPH scavenging activity, and metal ion chelating activity. This study compared these five extractsin terms of their inhibiting effects on three human malignant melanomas: A375, A375.S2, and A2058. MTT assay presented thenotion that both EA and DMextracts inhibited melanoma growth but did not affect the viabilities of normal dermal keratinocytes (HaCaT) or fibroblasts. Western blot analyses showed that both EA and DM extracts induced overexpression of caspase proteinsin all three melanomas. To determine their roles in melanogenesis, this study analyzed their in vitro suppressive effects onmushroomtyrosinase.All extracts except for water revealedmoderate suppressive effects.None of the extracts affected B16-F10 cellsproliferation. EA extract inhibited cellular melanin production whereas DMextract unexpectedly enhanced cellular pigmentationin B16-F10 cells. Data for modulations of microphthalmia-associated transcription factor, tyrosinase, tyrosinase-related protein 1and tyrosinase-related protein 2 showed that EA extract inhibited protein expression mentioned above whereas DMextract had theopposite effect. Overall, the experiments indicated that the biofunctional activities of EA extract contained in food and cosmeticsprotect against oxidation, melanoma, and melanin production.
Melanoma is the deadliest cancer. We identified 7-hydroxydehydronuciferine (7-HDNF) isolated from the leaves ofNelumbo nucifera Gaertn cv. Rosa-plena to be a bio-active agentthat antagonizes melanoma tumor growth in mice xenograftmodel in vivo. Cell proliferation assay demonstrated stronganticancer effects of 7-HDNF to exhibit a dose-dependentbehaviour and displayed minor cytotoxicities on normal humanskin cells, includingepidermal keratinocytes and melanocytes, and dermal fibroblasts. With acridine orange (AO) staining andflow analysis, we found 7-HDNF induced the formation ofintracellular vacuoles and the augmentation of acidic vesicularorganelles (AVO). The
apoptoticcell death ratio was measuredvia two-dimensional flow cytometry by annexin V-fluoresceinisothiocyanate (FITC)/propidium iodide (PI) double stained toconfirm the cellular membrane asymmetry lost. Onedimensionalflow cytometric analysis showed 7-HDNF increasedthe cellular arrest in cell cycle at the G2/M phase. ThroughWestern blot examinations, protein expressions were discoveredto verify autophagy and apoptosis response mechanisms sharingthe associated pathways. Finally, 7-HDNF presented ahigh-quality antimigratory activity in wound-healing assay. Overall, 7-HDNF presented high-quality anticancerbio-functions and inhibited melanoma tumor growth in vivoand in vitro.
Bromodomain-containing protein 4 (BRD4) has recently emerged as an attractive epigenetic target foranticancer therapy. In this study, an iridium(III) complex is reported as the first metal-based, irreversibleinhibitor of BRD4. Complex 1a is able to antagonize the BRD4-acetylated histone protein–proteininteraction (PPI) in vitro, and to bind BRD4 and down-regulate c-myc oncogenic expression in cellulo.Chromatin immunoprecipitation (ChIP) analysis revealed that 1a could modulate the interactionbetween BRD4 and chromatin in melanoma cells, particular at the MYC promoter. Finally, thecomplex showed potent activity against melanoma xenografts in an in vivo mouse model. To ourknowledge, this is the first report of a Group 9 metal complex inhibiting the PPI of a member of thebromodomain and extraterminal domain (BET) family. We envision that complex 1a may serve as auseful scaffold for the development of more potent epigenetic agents against cancers such asmelanoma.
Three new butanolides, isophilippinolide A, philippinolide A, and philippinolide B, and an amide, cinnaretamine, were isolated from the roots of Cinnamomum philippinense to be identified by spectroscopic analysis. Four isolated compounds were screened to examine their radical-scavenging ability, metal-chelating power, and ferric-reducing antioxidant power assay (FRAP). Cinnaretamine showed powerful antioxidative properties in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay and a reducing activity; all compounds presented minor inhibition of metal-chelating capacities. The effects of anti-tyrosinase of C. philippinense constituents were determined by the level of the suppression of hydroxylation that turned from L-tyrosine to L-dopathrough an in vitro mushroom tyrosinase assay, and all testing samples illustrated slight mushroom tyrosinase inhibitoryproperties. Isophilippinolide A exhibited inhibitory effectivenesses against the A375.S2 melanoma cell line in a cell viability assayat concentrations ranging from 0 to 200 μM for 24 h. Propidium iodide staining and flow cytometry analyses were applied toassess cell cycle accumulative distribution. It was discovered that isophilippinolide A caused sub-G1 phase accumulation inpositive correlation for apoptosis to inhibit cell growth. Further investigation revealed that isophilippinolide A induced A375.S2cells with an increase of caspase-dependent apoptotic proteins to trigger correlated pathway mechanisms according to Western
blotting results. Finally, isophilippinolide A displayed only low cytotoxicities to human normal epidermal cells (melanocytes) anddermal cells (fibroblasts). Altogether, the results implied C. philippinense compounds could be considered functional ingredientsin cosmetics, foods, and pharmaceutical products, particularly for their anticancer ability on human skin melanoma cells.
Kinetically inert metal complexes have arisen aspromising alternatives to existing platinum and rutheniumchemotherapeutics. Reported herein, to our knowledge, is thefirst example of a substitutionally inert, Group 9 organometalliccompound as a direct inhibitor of signal transducer andactivator of transcription 3 (STAT3) dimerization. Froma series of cyclometalated rhodium(III) and iridium(III)complexes, a rhodium(III) complex emerged as a potentinhibitor of STAT3 that targeted the SH2 domain and inhibitedSTAT3 phosphorylation and dimerization. Significantly, thecomplex exhibited potent anti-tumor activities in an in vivomouse xenograft model of melanoma. This study demonstratesthat rhodium complexes may be developed as effective STAT3inhibitors with potent anti-tumor activity.
University of veterinary and animal sciences,Pakistan
Muhammad Khurram Waqas is an Assistant Professor in Pharmaceutics in Institute of Pharmaceutical Sciences, University of Veterinary And Animal Sciences, Lahore, Pakistan. He earned his PhD degree from the Islamia University of Bahawalpur, Pakistan in 2015. The topic of his PhD Dissertation was “Formulation development and non-invasive in vivo evaluation of cosmetic emulsions containing various botanical extracts”. He has published over 20 research articles on cosmeceuticals sciences, health sciences, natural products and clinical research. His research interest including anti aging, transdermal drugs, cosmetics (safety, efficacy and quality control), hydrogels and reactive oxygen species.
The demand for botanical extracts in cosmeceuticals is growing day by day. Botanical extracts that support the skin integrity; vigour and texture are extensively used in cosmetics. Phytotherapy has been applied for decades to manage conditions of the skin and miscellaneous dermatological ailments consisting of Acne, Eczema, Inflammation, Psoriasis and Phototoxicity. The present paper highlights the applications of Vitis vinifera seeds extract in cosmetic formulations due to its minor toxicity with a short overview of the active constituents responsible for effective outcomes. The applications of grape seeds extract in dermatological care e.g. Stimulation of Collagen Synthesis, Protection of skin from UV radiations, anti-inflammatory, Skin lightening effects, free radical neutralizing effects has been elaborated. This is an effort to give brief overview on cosmetic impacts of grape seeds and its major components
Marjan Farshadi has her expertise in science-based natural remedies. After years of experience in research, natural health, medicine and pharmaceuticals, she established a research-based company to continue in-depth research and development of natural health products based on scientific evidences. Increasing interest in natural health remedies, weak regulations and manufacturing of natural health products by non-science-based settings can lead to serious conditions. She is determined to fill the gap and connect natural health products to science.
Wound healing is a complex process that involves several phases that range from coagulation, inflammation, accumulation of fundamental elements, to proliferation, formation of fibrous tissues and collagen, contraction of wound and formation of granulation tissue and scar.
Rhizome of Curcuma longa L. (common name: turmeric), the main spice of curry, has been used traditionally as a wound healing agent. Researchers around the world have studied the effect of curcumin, the most active ingredient of turmeric, for many years due to its bio-functional properties, especially antioxidant, radical scavenger, antimicrobial and anti-inflammatory activities, which play a critical role in the wound healing process. Furthermore, curcumin stimulated the production of the growth factors involved in the wound healing process, and so curcumin also enhanced the management of wound restoration.
Studies have provided evidence of the ability of curcumin to reduce the body's natural response to cutaneous wounds such as inflammation and oxidation. The recent literature on the wound healing properties of curcumin have also shown that curcumin has the ability of enhancing granulation tissue formation, collagen deposition, tissue remodeling and wound contraction. It has also been reported that curcumin may modulates wound healing in vitro in a biphasic dose response manner i.e. may be stimulatory at low doses and inhibitory at higher doses. Curcumin was reported to facilitate fibrinolysis and cellular migration during wound healing by modifying urokinase plasminogen activator expression.
Studies to date have shown that compared to its oral administration, topical application of curcumin has more noticeable effects on wound healing due to the greater accessibility of the drug at the wound site.
I will be reviewing the effect of curcumin on the wound healing, proposed mechanisms of action, and different curcumin-based cutaneous products and their effect on the wound healing.
4Tel Aviv University, Israel
Zamir H is currently working in the Departments of Dermatology, Rambam Health Care Campus and the Bruce Rappaport Faculty of Medicine, Haifa, Israel
Background: PDM is a rare variant of melanoma. It is confined to the dermis and/or subcutis without epidermal component. Hence, histologically alone, it cannot be distinguished from a melanoma metastasis. Careful clinical history, examination and proper imaging are required to exclude metastatic disease. Diagnosing such a lesion as PDM or as a metastasis has significant impact on the proper management and on the prognosis.
Our experience and insights cast some light on this problematic clinical situation.
Methods: We retrieved all 36 clinically diagnosed PDM patients from our prospectively collected database of 1600 melanoma patients surgically treated in a tertiary melanoma center between 1995 and 2015. Strict focused pathology revision excluded 23 patients who failed to meet all criteria for PDM set forth by Swetter et al. 13 patients were in-depth investigated regarding clinical, histopathological parameters, surgical treatment and outcome.
Results: 13 PDMs, median "thickness" – 5.5 (range 2 to 11) mm, median mitotic rate 4 (range 0 to 17)/mmsq, formed this series.11 pts (84.6%) are still alive after median follow up of 56 (range 12 to 120) months. 3 of 13 patients had a positive sentinel lymph node followed by completion dissection.
It seems that when PDM is diagnosed, it can be successfully treated as a primary melanoma (rather than a metastasis) including Sentinel node sampling and completion dissection
The actual "thickness" of these melanomas, as well as their mitotic rates, are associated with better prognosis than same-thickness cutaneous melanomas.
Conclusions: Diagnosing PDM demands careful clinical and histological reviews. PDM has favorable outcome compared to primary cutaneous same –thickness melanoma. Wide local excision and Sentinel lymph node biopsy seem appropriate and worthwhile, once these lesions are deemed primary melanomas
Center for aesthetic surgery, Croatia
Ass. Prof. Zoran Žgaljardić M.D., Ph.D. is an maxillofacial surgery consultant, head and nesck plastic surgery consultant and general cosmetic surgeon - international of International board of cosmetic surgery (IBCS). He is the Board member of Academy of Cosmetic Surgery, Croatian representative in International Academy of Cosmetic surgery and EUSAS Board ( Europen Society of Aesthetic Surgery).He is also one of the co-founders of World Academy of Cosmetic Surgery.He is Pionir in laser liposuction and invited speaker in workshops in lasers lipolysis.
Laser lipolysis with 1470 nm can be done as a procedure for itself but in most cases is followed by liposuction. The main goal of this procedure is to dissolve unwanted fat.
The secondary effect of this technology is skin tightening which is direct consequence of the photothermal effect which stimulates neocolagenesis.Target for the 1470 nm wave length water molecules. By adding infiltration of Klein's solution in the subcutaneous layer and fat subdermal tissue both effects are higher and the results are more evident.Technique is atraumatic and less painful with minor hematomas and edema of the skin than after standard liposuction. Laser lipolysis of the facial area can be done without subsequent liposuction. The result is lifting effect of the skin so we can call lit endolight lifting and can be done as an initial part of surgical lifting or as an isolated procedure.When laser lipolysis is used in „big“ liposuction (5000 ml) the aspiration of the fat content is more dense comparing with standard liposuction which means that really more fat is removed because it was dissolved before aspiration- liposuction.Subdermal region is stimulated with energy and that improves skin texture and final adaptation of skin because of new collagen formation
The aim of the study is to determine effects of Skin Promotion Program (SPP) in reducing occupational skin diseases symptoms and improvement of protective health behaviors in young workers who are hairdresser/barber apprentice at vocational training centers. The study was designed as pre- and post-test control group research with repetitive measurement, and consisted of 100 young workers (Intervention=50, control=50 ) with a score of 2 points or less on eczema symptom, educated and who were of two different. The SPP program was developed on the basis of the health promotion model that applied to intervention group. The program consists of evidence-based education and counseling practices aimed at improving health and preventive health behaviors (hand washing, using latex-free (nitrile) gloves, cotton gloves and moisturizers). Both groups were examined by a specialist physician to determine the degree of eczema symptoms before and after intervention. The monitoring processes were made with workplace health surveillance, message, telephone call, WhatsApp messages, check-lists and photo records. The data collection tools were; dermatology life quality index, self-efficacy scale, Turkish Nordik occupational skin questionnaire, eczema symptom scale assessment form, protective behavior monitoring form. The mean age of young workers was found to be 20.77±4.85. After the program, there was a decrease in favor of the intervention group at the eczema symptoms (redness, itching, dryness, cracking/splitting, flake/scaling and total eczema) score between the both groups. Also; within the intervention group, there was a statistically significant difference between the scores of eczema symptom and skin protection behaviors before and after the program (p˂0.05). The SPP is effective in reducing of eczema symptoms and improving preventive health behaviors and self-efficacy of young workers who work as hairdresser/berbers
Emine Kurtulus is a Research Assistant at Istanbul University - Florence Nightingale Faculty of Nursing, Public Health Nursing department. She completed her Graduation at Istanbul University Institute of Health Sciences. She has five articles in international and national journals and has been serving as a Journal Referee. Her research interest is in the area of “Occupational skin diseases in young workers, apprentice skin diseases”.